against pathogens affecting both cold and warm water aquatic species
such as
Aeromonas hydrophila
,
Aeromonas salmonicida, Yersinia ruckeri
and
Vibrio parahameolyticus
(Nuez-Ortín, 2011, Unpublished data)
.
At the same
time, this inhibitory activity reduces nutrient competition and pathogen
colonization of the gut resulting in improved absorptive surface area. It is
also known that probiotic bacteria can release extracellular enzymes that
help in the digestion process. In a study conducted by the University of
Sonora (Mexico) the protease activity in hepatopancreas of shrimp was
assessed when Ecobiol Aqua was supplemented at 1 kg/ton of feed (Figure
2). Results showed increased enzymatic activity during the first four hours
after feed intake, suggesting enhanced digestibility and consequently
better growth performance. Secretion of amylases, cellulases and xylanases
has also been reported for
B. amyloliquefaciens
(Cortyl, 2010).
Table 2.
Ecobiol Aqua
survival after processing (90°C during 1 minute)
Mash (UFC/g feed)
Pellet (UFC/g feed)
2.60 x 10
6
2.25 x 10
6
1.68 x 10
6
1.50 x 10
6
1.27 x 10
6
1.36 x 10
6
The efficacy of dietary supplementation of Ecobiol Aqua on performance
parameters at a commercial scale has been evaluated in shrimp cultured in
Mexico. In a first trial carried out in a semi-intensive farm, diets formulated
for
Litopenaeus vannamei
were supplemented with 1 kg Ecobiol Aqua per
ton of feed. As shown in Table 3, results showed improvements in survival
(+3.7%), average weight gain at 95 days (+4%), and income (+12%) relative
to the non-supplemented diet. The same dosage was used in a second trial
conducted in semi-intensive culture of
Litopenaeus vannamei
(Table 3),
leading to better average weekly gain (+13.5%) and income (+7.2%) with
Ecobiol Aqua supplementation.
Therefore, the use of
Bacillus
spp. is common in aquaculture practices, both
in feed and in water. There is a number of studies showing that
Bacillus
Figure 2. Enzymatic
and growing response
of
Litopenaeus
vannamaei
to a diet
containing ECOBIOL
Aqua
14
Natural growth promoters in aquaculture practices
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